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VITAMIN B1 (THIAMINE)
Number 1379
CPT Code 84425
Alias THIAMINE
Specimen Container Lavender Top
Specimen Type Whole Blood EDTA
Turn Around Time
Days Performed
Reference Range 66.5-200.0 nmol/L
Alternate Specimen
Volume 1 mL Whole Blood
Stability Ambient Unstable
Stability Refrigerated 2 Days
Stability Frozen 7 Days
Patient Preparation
Methodology
High-pressure liquid chromatography (HPLC)
Limitations
The biologically active form of the vitamin, thiamine diphosphate (TDP), is best measured in whole blood and is not found in measurable concentration in plasma. Plasma thiamine concentration reflects recent intake rather than body stores; therefore, whole blood is the preferred specimen for thiamine assessment. Approximately 80% of thiamine present in whole blood is found in red blood cells.
Rejection Criteria
Specimen not frozen (-20°C); plasma specimen; use of anticoagulants other than EDTA; specimen not protected from light
Additional Instructions
Protect from light
Performing Laboratory Reference Laboratory
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