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CHROMOSOME ANALYSIS, BLOOD
Number 174
CPT Code 88262
Alias CHROMOSOME ANALYSIS
Specimen Container Green Top Heparin
Specimen Type Whole Blood Sodium Heparin
Turn Around Time 
Days Performed 
Reference Range 
Alternate Specimen 
Volume 5 mL Whole Blood
Stability Ambient 
Stability Refrigerated 
Stability Frozen N/A
Patient Preparation 

Pertinent medical findings must accompany request for chromosome analysis. Include the patient’s name, age and suspected diagnosis.

Methodology 

Lymphocyte culture with mitogen stimulation, colchicine arrest of cells in mitosis with predominantly early metaphases, methanol/acetic acid fixation, spread preparation, trypsin-Giemsa banding, chromosome analysis of 20 metaphases with preparation and analysis of multiple karyotypes.

Limitations 
Rejection Criteria 

Hemolysis; clotted specimen; use of improper anticoagulant; frozen specimen

Additional Instructions 
Performing Laboratory Reference Laboratory
TagsKARYOTYPE, G-BANDING, CONSTITUTIONAL