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Adult patients should be on a standard diet containing 50 to 150 g of fat per day for at least three days before test is started and during the 72-hour collection. In children, the amount of fat in the diet should be constant for one day before the test and during the test. The patient should not have had mineral oil as a laxative prior to specimen collection.
C8 and C10 saturated fatty acids are not quantitated with this method.
Container more than 2/3 full; container leaking; improper container; ); foreign matter other than feces inside of container (ie, spoons, tongue depressors, plastic bags, toilet paper, etc); patient not on special diet; improper labeling of container or test request form; specimen on outside of container; specimen contaminated with urine; aliquot without “net weight” and “amount of water added” to homogenize the sample
Container and request form must indicate collection time (in hours). Collection of 24 or 48 hours is not recommended since results are subject to greater variability. If sending Aliquot of sample, request form must indicate weight of entire specimen collected.