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High-pressure liquid chromatography (HPLC)
The biologically active form of the vitamin, thiamine diphosphate (TDP), is best measured in whole blood and is not found in measurable concentration in plasma. Plasma thiamine concentration reflects recent intake rather than body stores; therefore, whole blood is the preferred specimen for thiamine assessment. Approximately 80% of thiamine present in whole blood is found in red blood cells.
Specimen not frozen (-20°C); plasma specimen; use of anticoagulants other than EDTA; specimen not protected from light
Protect from light